| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,373,368 | G→A | 100% | R480H (CGT→CAT) | ycjM → | predicted glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,373,368 | 0 | G | A | 83.4% | 114.9 / 15.6 | 48 | R480H (CGT→CAT) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/1); new base A (14/26); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCG > W3110S.gb/1373307‑1373437 | aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:160368/1‑71 (MQ=255)aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:936298/1‑71 (MQ=255)aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:424037/1‑71 (MQ=255)aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:345590/1‑71 (MQ=255)aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:830109/1‑71 (MQ=255) tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata < 1:1587204/71‑1 (MQ=255) tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata < 1:866826/71‑1 (MQ=255) tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata < 1:1940631/71‑1 (MQ=255) tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata < 1:683685/71‑1 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1877324/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1651026/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:595738/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1447961/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1292422/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1289564/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1941768/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:213478/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1050199/1‑71 (MQ=255) tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:214551/71‑1 (MQ=255) tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:1474412/71‑1 (MQ=255) atatCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGataa > 1:1418211/1‑63 (MQ=255) atatCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTa > 1:1809287/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATca > 1:1574103/1‑53 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:510270/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1533243/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1378389/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATCTTACCa > 1:962453/1‑71 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:1552270/71‑1 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:287556/71‑1 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:1228162/71‑1 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata < 1:1056540/50‑1 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTa < 1:116143/71‑1 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTa < 1:731224/71‑1 (MQ=255) aaTTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa < 1:91240/70‑1 (MQ=255) aaTTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa < 1:747610/70‑1 (MQ=255) aaTTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa < 1:60246/70‑1 (MQ=255) ttACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt < 1:936243/66‑1 (MQ=255) ttACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt < 1:4414/66‑1 (MQ=255) ttACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt < 1:737035/66‑1 (MQ=255) ttACACTTCATCGCAGCCATAACGAGTTTCATCC‑‑ATAATAATTTTACCATTGATACGATTAAtt < 1:975016/64‑1 (MQ=255) acacTTCATCGCAGCCA‑AACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1435208/70‑1 (MQ=255) cacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1527969/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1420936/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:884272/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1611018/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1683544/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:170237/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1109009/70‑1 (MQ=255) gtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTTATGCg < 1:1240412/70‑1 (MQ=255) gtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCg < 1:1794970/70‑1 (MQ=255) | AACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCG > W3110S.gb/1373307‑1373437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |