Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,436,722 | T→C | 17.4% | intergenic (‑751/+177) | ynaE ← / ← uspF | predicted DNA‑binding transcriptional regulator/stress‑induced protein, ATP‑binding protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,436,722 | 0 | T | C | 17.4% | 108.5 / 15.9 | 46 | intergenic (‑751/+177) | ynaE/uspF | predicted DNA‑binding transcriptional regulator/stress‑induced protein, ATP‑binding protein |
Reads supporting (aligned to +/- strand): ref base T (11/27); new base C (4/4); total (15/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCCCTTACTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGT > W3110S.gb/1436664‑1436767 | tGCCCTTACTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACACTTAAAAGCGAc < 1:1888213/70‑1 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAAtt > 1:189669/1‑69 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:961991/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:812206/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:537296/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:479607/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:406647/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:1804262/1‑71 (MQ=255) aCTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCg > 1:1752166/1‑71 (MQ=255) cTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt < 1:1133486/71‑1 (MQ=255) cTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt < 1:1159826/71‑1 (MQ=255) cTTGTTCAGGTTGTATTGTCCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt < 1:1053724/71‑1 (MQ=255) gTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:996598/70‑1 (MQ=255) gTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:846694/70‑1 (MQ=255) gTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:339963/70‑1 (MQ=255) gTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:1262861/70‑1 (MQ=255) gTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:1294098/70‑1 (MQ=255) gTTCAGGTGGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:1832704/70‑1 (MQ=255) ttCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCGTtat < 1:164650/70‑1 (MQ=255) ttCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCGTtat < 1:985759/70‑1 (MQ=255) ttCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCGTtat < 1:389962/70‑1 (MQ=255) tGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt > 1:834082/1‑60 (MQ=255) tGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt > 1:1722237/1‑60 (MQ=255) tGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt > 1:453384/1‑60 (MQ=255) tGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATgg < 1:1332907/62‑1 (MQ=255) tGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATgg < 1:1512058/62‑1 (MQ=255) ttctttctTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:851651/71‑1 (MQ=255) ttctttctTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:467531/71‑1 (MQ=255) ttctttctTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:1435937/71‑1 (MQ=255) ttctttctTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:1458081/71‑1 (MQ=255) ttctttctTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:1545452/71‑1 (MQ=255) tctttcttACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAagag < 1:1567116/70‑1 (MQ=255) tcttACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTa < 1:425588/68‑1 (MQ=255) cttACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGc < 1:697222/70‑1 (MQ=255) cttACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGc < 1:128632/70‑1 (MQ=255) tACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGt < 1:674965/70‑1 (MQ=255) aCTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGt < 1:1736216/69‑1 (MQ=255) cTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGt < 1:1546901/68‑1 (MQ=255) tAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTa < 1:1549931/62‑1 (MQ=255) aTTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCg > 1:237030/1‑39 (MQ=255) aTTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTa > 1:1133605/1‑60 (MQ=255) aTTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTa > 1:1621183/1‑60 (MQ=255) aTTTCTTGATTTTGCGACACTTAAAAGCGACTCAATTCATTATATGGCATCAGAAGAGTa > 1:1101117/1‑60 (MQ=255) tttCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGt < 1:781152/39‑1 (MQ=255) tGATTTTGCGACATTTAAAAGCGACTCAATTCGTta < 1:1576756/36‑1 (MQ=255) aTTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGt < 1:1770425/47‑1 (MQ=255) | TGCCCTTACTTGTTCAGGTTGTATTGTTCTTTCTTACTAATTTCTTGATTTTGCGACATTTAAAAGCGACTCAATTCGTTATATGGCATCAGAAGAGTATGCGT > W3110S.gb/1436664‑1436767 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |