Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,521,063 C→T 100% S108F (TCC→TTC)  yncB → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,521,0630CT100.0% 25.1 / NA 10S108F (TCC→TTC) yncBpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/10);  total (0/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTG  >  W3110S.gb/1521044‑1521127
                   |                                                                
tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCg                                     <  1:1825130/49‑1 (MQ=255)
tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCg                                     <  1:29333/49‑1 (MQ=255)
 ggCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGTCg                                     <  1:1573771/48‑1 (MQ=38)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:1115016/71‑1 (MQ=255)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:1583101/71‑1 (MQ=255)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:1861353/71‑1 (MQ=255)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:1863755/71‑1 (MQ=255)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:1906523/71‑1 (MQ=255)
             aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:423699/71‑1 (MQ=255)
              cATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg  <  1:236709/70‑1 (MQ=255)
                   |                                                                
TGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTG  >  W3110S.gb/1521044‑1521127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑