| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,569,965 | T→C | 100% | Q191Q (CAA→CAG) | yddW ← | predicted liprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,569,965 | 0 | T | C | 81.6% | 88.4 / 12.8 | 38 | Q191Q (CAA→CAG) | yddW | predicted liprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base C (14/17); total (14/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGA > W3110S.gb/1569903‑1570017 | aCCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa < 1:1280574/71‑1 (MQ=255)aCCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa < 1:946773/71‑1 (MQ=255)aCCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa < 1:357621/71‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:1075915/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:975693/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:95618/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:899500/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:1972898/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGca < 1:1695376/57‑1 (MQ=255) tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCAAGTCGCGGTGTTGca < 1:467048/57‑1 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCTGTGCTGCACATAGACGCTCGCCgg > 1:437025/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:1297116/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:1525235/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:844573/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:532977/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:1402280/1‑71 (MQ=255) cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg > 1:356917/1‑71 (MQ=255) ccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgt < 1:738173/71‑1 (MQ=255) tCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgttgtt > 1:995902/1‑70 (MQ=255) tCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgttgt > 1:861938/1‑69 (MQ=255) gACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgttg > 1:914720/1‑63 (MQ=255) gACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgttg > 1:1459487/1‑63 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:381970/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:1201470/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:1495113/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:1351964/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:1656184/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:1471943/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:49996/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:386759/71‑1 (MQ=255) aCAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACag < 1:34807/71‑1 (MQ=255) cGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGgt < 1:1532213/54‑1 (MQ=255) ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTgag < 1:893737/48‑1 (MQ=255) ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTgag < 1:1034817/48‑1 (MQ=255) cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa > 1:726433/1‑70 (MQ=255) cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa > 1:52363/1‑70 (MQ=255) cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa > 1:299876/1‑70 (MQ=255) cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa < 1:1478447/70‑1 (MQ=255) | ACCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGA > W3110S.gb/1569903‑1570017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |