| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,588,010 | T→G | 48.0% | D64A (GAC→GCC) | ydeP ← | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,588,010 | 0 | T | G | 48.0% | 1.4 / 40.1 | 25 | D64A (GAC→GCC) | ydeP | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (9/4); new base G (5/7); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGAAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAT > W3110S.gb/1587941‑1588080 | aagaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTc > 1:1127211/1‑71 (MQ=255)aagaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTc > 1:570659/1‑71 (MQ=255)aagaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTc > 1:196718/1‑71 (MQ=255)aagaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTc > 1:1950529/1‑71 (MQ=255) aagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTT‑CGCCGTTTTCACAAATGGCGAAt > 1:1360857/1‑70 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACtgt > 1:391960/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACtgt > 1:1761527/1‑71 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGAt > 1:279247/1‑71 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGAt > 1:411950/1‑71 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGAt > 1:1832991/1‑71 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1081013/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGc < 1:336646/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTACTTAGGATCTGGc < 1:500803/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1658998/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1461063/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGc < 1:581130/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1180943/71‑1 (MQ=255) tGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTAACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1715888/71‑1 (MQ=255) aCTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1721988/69‑1 (MQ=255) cccAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCAATg < 1:726054/69‑1 (MQ=255) tGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCa < 1:60250/70‑1 (MQ=255) ttCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:760085/1‑49 (MQ=255) ttCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:1720634/1‑49 (MQ=255) ttCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:1455899/1‑49 (MQ=255) ttCACAAATGGCGAATGAAGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:420981/1‑49 (MQ=39) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:753853/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:828080/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:69253/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:601254/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:500301/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:1370601/1‑71 (MQ=255) tCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCAGACAGTCAAAGCCCTCTGGCTTATTCAt > 1:1216205/1‑71 (MQ=255) | AAGAAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCAT > W3110S.gb/1587941‑1588080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |