BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,742,822	A→G	63.0%	S400G (AGC→GGC)	ydhC →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,742,822	0	A	G	63.0%	20.7 / 25.6	27	S400G (AGC→GGC)	ydhC	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (8/2); new base G (12/5); total (20/7)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

GGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTATG  >  W3110S.gb/1742756‑1742886

ggTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCg                                                               <  1:27323/70‑1 (MQ=255)

ggTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCg                                                               <  1:1266766/70‑1 (MQ=255)

ggTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCg                                                               <  1:1919706/70‑1 (MQ=255)

ggTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCg                                                               <  1:663552/70‑1 (MQ=255)

            cAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCGAATCACACTGATc                                                  >  1:1342039/1‑71 (MQ=255)

                          agTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGAtatact                                    <  1:531228/71‑1 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACCTATACTTAGgctgc                      >  1:1477003/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACCTATACTTAGgctgc                      >  1:1581878/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:157216/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:1674898/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:752425/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:1301268/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:1267630/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:1267543/1‑70 (MQ=255)

                                         tCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGgctgc                      >  1:924762/1‑70 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGATTCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAGAAttt           >  1:1754991/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:1174399/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:839613/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:1750163/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:161566/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:1383305/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:1147898/1‑71 (MQ=255)

                                                   gCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAAttt           >  1:548660/1‑71 (MQ=255)

                                                          tCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTa    >  1:314176/1‑71 (MQ=255)

                                                          tCGCTCATGGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTa    >  1:805680/1‑71 (MQ=255)

                                                            gCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTATg  <  1:556961/71‑1 (MQ=255)

                                                            gCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTATg  <  1:1730967/71‑1 (MQ=255)

GGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCATAGCGAATCACACTGATCTATATCGATATACTTATACTTAGGCTGCTAACAAAATTTTGTTGTATG  >  W3110S.gb/1742756‑1742886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`