| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,775,080 | T→C | 100% | F285F (TTT→TTC) | ydiN → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,775,080 | 0 | T | C | 91.7% | 32.9 / ‑3.4 | 12 | F285F (TTT→TTC) | ydiN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (7/4); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCA > W3110S.gb/1775020‑1775134 | tgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTg < 1:1802779/68‑1 (MQ=255) ttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAAt < 1:1787369/70‑1 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCa > 1:1038443/1‑71 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCa > 1:1873541/1‑71 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCa > 1:796648/1‑71 (MQ=255) tCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg < 1:1204251/59‑1 (MQ=255) tCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg < 1:1569173/59‑1 (MQ=255) tCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTg < 1:1648152/59‑1 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa > 1:1269190/1‑59 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa > 1:657723/1‑59 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa > 1:920771/1‑59 (MQ=255) tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCa > 1:940324/1‑59 (MQ=255) | TGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCA > W3110S.gb/1775020‑1775134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |