| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,793,663 | G→A | 17.0% | V24V (GTC→GTT) | ydiV ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,793,663 | 0 | G | A | 17.0% | 131.7 / 18.8 | 53 | V24V (GTC→GTT) | ydiV | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/31); new base A (4/5); total (17/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
ATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTT > W3110S.gb/1793621‑1793731 | aTCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:910437/71‑1 (MQ=255)aTCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGGTGTCTCTTATCGGCAGGa < 1:1776917/71‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:9990/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:869157/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1917015/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1773339/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:344047/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1707096/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1574730/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1529404/70‑1 (MQ=255) tCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGa < 1:1351231/70‑1 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGaaa > 1:204330/1‑70 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGaaa > 1:1614570/1‑70 (MQ=255) ttCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGaaa > 1:1746803/1‑70 (MQ=255) cACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:311349/1‑71 (MQ=255) cACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:1659544/1‑71 (MQ=255) cACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:335641/1‑71 (MQ=255) cACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAg > 1:1150323/1‑71 (MQ=255) cTGGAGAAATGGGTAATTAACTCAACGCCGATAAGAACTTGCTg < 1:1847770/44‑1 (MQ=38) cTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTg < 1:476601/44‑1 (MQ=255) cTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTg < 1:757755/44‑1 (MQ=255) cTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTg < 1:1638643/44‑1 (MQ=255) cTGGAGAAATGGGTAATTAACTCAACGCCGACAAGAACTTGCTg < 1:1432676/44‑1 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:854735/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:602124/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:1823115/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:319542/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:1589115/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:310475/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:1117525/1‑71 (MQ=255) aTGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAAt > 1:908203/1‑71 (MQ=255) aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATaaa < 1:476102/70‑1 (MQ=255) aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATaaa < 1:849297/70‑1 (MQ=255) aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATaaa < 1:1038727/70‑1 (MQ=255) aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATaaa < 1:1749853/70‑1 (MQ=255) aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATaaa < 1:1265028/70‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:1887340/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:1876665/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:1783297/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:348845/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:351176/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:1420532/71‑1 (MQ=255) ttAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGa < 1:566925/71‑1 (MQ=255) aaCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGAt > 1:1727320/1‑70 (MQ=255) aaCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGAt > 1:1332784/1‑70 (MQ=255) tCAACGCCGACAAGGACTTGCTGGGTGTCTCTTATCGGCAGGa < 1:935892/43‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:1861681/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:732872/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:277752/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:264310/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:138714/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:1127187/71‑1 (MQ=255) aGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCtt < 1:1063053/71‑1 (MQ=255) | ATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTT > W3110S.gb/1793621‑1793731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |