| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,832,108 | C→T | 52.2% | T124T (ACG→ACA) | astA ← | arginine succinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,832,108 | 0 | C | T | 52.2% | ‑2.3 / 29.6 | 23 | T124T (ACG→ACA) | astA | arginine succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (7/4); new base T (9/3); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGT > W3110S.gb/1832040‑1832166 | aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGt > 1:1561924/1‑71 (MQ=255)aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGt > 1:699772/1‑71 (MQ=255) ataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGca < 1:754569/71‑1 (MQ=255) taaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGca < 1:1067993/70‑1 (MQ=255) taaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGca < 1:897675/70‑1 (MQ=255) ttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct > 1:1601720/1‑71 (MQ=255) ttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct > 1:657655/1‑71 (MQ=255) ttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgc > 1:1613032/1‑70 (MQ=255) tCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGctgctg > 1:1242556/1‑71 (MQ=255) tCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGctgctg > 1:1693527/1‑71 (MQ=255) gCCGTTGCCCTCTTTGCGCCAGTCCGGGTGAAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:484025/71‑1 (MQ=255) gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGGTCGCTGCTGCCGGTGTGATCGTTACt > 1:696645/1‑69 (MQ=255) gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTAc > 1:1438801/1‑68 (MQ=255) gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACt > 1:591774/1‑69 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCtgctgc > 1:1803648/1‑35 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt > 1:23079/1‑70 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATg > 1:1874806/1‑71 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATg > 1:423837/1‑71 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATg > 1:1733466/1‑71 (MQ=255) cGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATg > 1:700244/1‑71 (MQ=255) gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt < 1:398581/71‑1 (MQ=255) gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt < 1:1487908/71‑1 (MQ=255) cAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt < 1:646797/62‑1 (MQ=255) | AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGT > W3110S.gb/1832040‑1832166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |