Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,927,287 T→C 100% L45P (CTT→CCT)  yobB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,927,2870TC85.7% 33.4 / ‑0.3 14L45P (CTT→CCT) yobBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (12/0);  total (12/2)
Fisher's exact test for biased strand distribution p-value = 1.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT  >  W3110S.gb/1927244‑1927349
                                           |                                                              
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:1369421/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:1466375/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:160484/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:1789010/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:1953265/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:426846/1‑70 (MQ=255)
gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                      >  1:723897/1‑70 (MQ=255)
     cgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg                                <  1:1101708/71‑1 (MQ=255)
                 aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                    >  1:1397400/1‑71 (MQ=255)
                 aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                    >  1:574971/1‑71 (MQ=255)
                 aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                    >  1:808895/1‑71 (MQ=255)
                                 tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt    >  1:1189555/1‑71 (MQ=255)
                                 tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt    >  1:439239/1‑71 (MQ=255)
                                    ccATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt  <  1:1698798/70‑1 (MQ=255)
                                           |                                                              
GCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT  >  W3110S.gb/1927244‑1927349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: