Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,927,287 | T→C | 100% | L45P (CTT→CCT) | yobB → | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,927,287 | 0 | T | C | 85.7% | 33.4 / ‑0.3 | 14 | L45P (CTT→CCT) | yobB | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (12/0); total (12/2) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT > W3110S.gb/1927244‑1927349 | gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:1369421/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:1466375/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:160484/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:1789010/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:1953265/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:426846/1‑70 (MQ=255) gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc > 1:723897/1‑70 (MQ=255) cgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg < 1:1101708/71‑1 (MQ=255) aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc > 1:1397400/1‑71 (MQ=255) aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc > 1:574971/1‑71 (MQ=255) aTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc > 1:808895/1‑71 (MQ=255) tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt > 1:1189555/1‑71 (MQ=255) tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt > 1:439239/1‑71 (MQ=255) ccATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt < 1:1698798/70‑1 (MQ=255) | GCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT > W3110S.gb/1927244‑1927349 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |