| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,935,986 | C→T | 81.5% | M111I (ATG→ATA) | edd ← | 6‑phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,935,986 | 0 | C | T | 81.5% | 57.6 / 7.8 | 27 | M111I (ATG→ATA) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (18/4); total (20/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT > W3110S.gb/1935917‑1936053 | cATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATcacaca > 1:820331/1‑71 (MQ=255)cATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATcacaca > 1:1454875/1‑71 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:1518262/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:364360/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:71862/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:733323/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:1799427/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:883634/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:1333304/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:1309745/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:886048/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:978370/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAcc > 1:99084/1‑70 (MQ=255) aCAATTCCATTCCATCCTGCCCCTGGGAGACACCATCACATATCGCCGGAACACCGCCCGCAAc > 1:1909779/1‑64 (MQ=255) ttccattccaTCCTGCCCCTGGGTGACACCATCACATATCGCCGGAac < 1:1222805/48‑1 (MQ=255) ttccattccaTCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCg < 1:1677449/71‑1 (MQ=255) ttccattccaTCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCg < 1:181570/71‑1 (MQ=255) ttccattccaTCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCg < 1:714328/71‑1 (MQ=255) ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc > 1:266261/1‑71 (MQ=255) ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc > 1:1290289/1‑71 (MQ=255) acatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACAccgcc > 1:1111681/2‑47 (MQ=39) gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:1807836/1‑71 (MQ=255) gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:1334405/1‑71 (MQ=255) gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:1208061/1‑71 (MQ=255) gACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTAc > 1:785309/1‑71 (MQ=255) gACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTAc > 1:929807/1‑71 (MQ=255) tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAt < 1:682624/71‑1 (MQ=255) tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAt < 1:300438/71‑1 (MQ=255) tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAt < 1:1930961/71‑1 (MQ=255) acacaTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAttt > 1:959652/1‑71 (MQ=255) acacaTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:55193/1‑70 (MQ=255) acacaTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:1027778/1‑70 (MQ=255) acacaTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:873492/1‑70 (MQ=255) | CATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT > W3110S.gb/1935917‑1936053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |