| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,965,064 | G→A | 100% | I567I (ATC→ATT) | flhA ← | predicted flagellar export pore protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,965,064 | 0 | G | A | 83.3% | 69.8 / 8.3 | 30 | I567I (ATC→ATT) | flhA | predicted flagellar export pore protein |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (16/9); total (21/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTT > W3110S.gb/1965041‑1965122 | gTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATcgc > 1:953446/1‑70 (MQ=255) ttAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTgcg < 1:1090011/61‑1 (MQ=255) aaTTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCa > 1:372609/1‑61 (MQ=255) aaTTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCa > 1:888346/1‑61 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:297555/1‑46 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:1683325/1‑46 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:629492/1‑46 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:1621184/1‑46 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:158529/1‑46 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag > 1:1322088/1‑46 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:713746/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:757806/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:1466632/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:1763770/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:624570/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:1801440/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:546653/57‑1 (MQ=255) tgtgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCata < 1:1952315/57‑1 (MQ=255) tgGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACtt > 1:1160716/1‑71 (MQ=255) tgGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACtt > 1:1675230/1‑71 (MQ=255) tgGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACtt > 1:1568759/1‑71 (MQ=255) tgGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACtt > 1:1422149/1‑71 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:259383/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:533435/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:1935412/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:1782383/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:1499129/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:924393/1‑57 (MQ=255) tgGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATc > 1:1227620/1‑57 (MQ=255) tgGATCACTTTGAATGGGCGCATGTTCCGCCAGCGATTCGAGAATGGTGCGCATATc > 1:252108/1‑57 (MQ=255) | GTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTT > W3110S.gb/1965041‑1965122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |