| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,995,272 | T→C | 54.5% | S524S (TCA→TCG) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,995,272 | 0 | T | C | 54.5% | 2.5 / 26.7 | 22 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base C (10/2); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACG > W3110S.gb/1995222‑1995340 | gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:1085761/71‑1 (MQ=255)gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:1315854/71‑1 (MQ=255)gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:163729/71‑1 (MQ=255)gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:1828686/71‑1 (MQ=255)gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:22013/71‑1 (MQ=255)gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTaaa < 1:1076288/71‑1 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCt > 1:833251/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTg > 1:788945/1‑53 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:1959061/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:89664/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:653455/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:230247/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:1745947/1‑71 (MQ=255) aTCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCt > 1:1599504/1‑71 (MQ=255) cATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGc > 1:1798765/1‑71 (MQ=255) cATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGc > 1:1240335/1‑71 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa > 1:258791/1‑70 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa > 1:474111/1‑70 (MQ=255) tGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc < 1:1909671/43‑1 (MQ=255) tGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc < 1:781567/43‑1 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCAc > 1:1876222/1‑38 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:924923/71‑1 (MQ=255) | GCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACG > W3110S.gb/1995222‑1995340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |