| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,157,782 | T→C | 73.7% | L128S (TTG→TCG) | mdtB → | multidrug efflux system, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,157,782 | 0 | T | C | 73.7% | 65.2 / 23.8 | 38 | L128S (TTG→TCG) | mdtB | multidrug efflux system, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base C (18/10); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
ACATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAT > W3110S.gb/2157718‑2157845 | aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGa > 1:138795/1‑70 (MQ=255)aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAg > 1:189239/1‑71 (MQ=255)aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAg > 1:964183/1‑71 (MQ=255)aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAg > 1:267850/1‑71 (MQ=255)aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAg > 1:1366915/1‑71 (MQ=255)aCATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAg > 1:1360317/1‑71 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTTCGACCAACTTGTCGCCGAGCg < 1:510697/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCg < 1:1493663/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCg < 1:1691909/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:87498/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:85002/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:585818/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:449927/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:255777/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:1800399/71‑1 (MQ=255) aTTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:1905762/71‑1 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:1340801/71‑1 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:1127813/71‑1 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa > 1:40415/1‑71 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:289827/71‑1 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:1360748/71‑1 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa > 1:1337460/1‑71 (MQ=255) ttACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGa < 1:1111091/71‑1 (MQ=255) ccGAGCATGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCg < 1:34815/54‑1 (MQ=255) aaGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAg > 1:877308/1‑70 (MQ=255) aaGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAg > 1:385033/1‑70 (MQ=255) aaGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAg > 1:1665029/1‑70 (MQ=255) aaGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAg > 1:579997/1‑70 (MQ=255) cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt > 1:5152/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:452105/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:1352487/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:1306180/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:150046/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:446254/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:113084/1‑71 (MQ=255) gCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATcc > 1:1765156/1‑71 (MQ=255) aCCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGAt < 1:1392980/71‑1 (MQ=255) aaCTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAt < 1:1759713/71‑1 (MQ=255) | ACATTACCGCTCGATGTCGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAACCCGGCAGATCCGCCGATCAT > W3110S.gb/2157718‑2157845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |