| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,172,235 | C→T | 29.8% | pseudogene (42/447 nt) pseudogene (1636/2041 nt) |
gatR ← gatR ← |
DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087 DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,172,235 | 0 | C | T | 29.8% | 85.3 / 44.3 | 57 | pseudogene (42/447 nt) pseudogene (1636/2041 nt) | gatR gatR | DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087 DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498 |
| Reads supporting (aligned to +/- strand): ref base C (30/10); new base T (9/8); total (39/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACG > W3110S.gb/2172175‑2172304 | cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATAtttt > 1:698832/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATAtttt > 1:1260147/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATAtttt > 1:1247099/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:1115958/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:1940194/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:764232/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:762041/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAtttt > 1:63461/1‑71 (MQ=255)cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGT‑‑‑TAATCACCGTGATATTTTTgg > 1:1211684/1‑71 (MQ=255) tAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCg > 1:741993/1‑71 (MQ=255) aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt < 1:1600085/71‑1 (MQ=255) gTCTTTATTTTCGGAAAGGGCATACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:765580/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCACACGCTGCCGGGAGACTGTTGGTAATCACCGTGata > 1:794629/1‑58 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:13519/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:1233503/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:451282/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:1072216/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:658751/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:263051/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:229324/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:888441/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:207532/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:112124/1‑71 (MQ=255) gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCa > 1:1926549/1‑71 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt > 1:124231/1‑71 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt > 1:1572982/1‑71 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGATGGTAATCACTGTGATATTTTTGGCGGTCAt > 1:1909935/1‑71 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGATGGTAATCACTGTGATATTTTTGGCGGTCAt > 1:302372/1‑71 (MQ=255) tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGATGGTAATCACTGTGATATTTTTGGCGGTCAt > 1:298644/1‑71 (MQ=255) tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:1153734/1‑70 (MQ=255) tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:1174723/1‑70 (MQ=255) tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:53638/1‑70 (MQ=255) tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAtt > 1:1667296/1‑70 (MQ=255) aTTTTCGGAAAGGGCAAACGCTGCCTGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAAt < 1:121835/70‑1 (MQ=255) ttttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATcc < 1:782544/71‑1 (MQ=255) ttttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATcc < 1:649550/71‑1 (MQ=255) ttttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATcc < 1:836233/71‑1 (MQ=255) ggaaaGGCTGCCGGGAGACTGTTGGTAATCAGCGTGTTTTTTTTGGCGGTCATTAATCCTTCGGCTATAAg < 1:780789/69‑1 (MQ=255) gCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg < 1:825801/71‑1 (MQ=255) gCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg < 1:1400924/71‑1 (MQ=255) gCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg < 1:855627/71‑1 (MQ=255) cAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:1270655/71‑1 (MQ=255) ccGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg < 1:1676199/61‑1 (MQ=255) gTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCgag < 1:51998/71‑1 (MQ=255) gTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCgag < 1:1148011/71‑1 (MQ=255) gTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCgag < 1:1395609/71‑1 (MQ=255) tCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:405832/44‑1 (MQ=255) tCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:425269/44‑1 (MQ=255) tCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:1134980/44‑1 (MQ=255) tCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc < 1:76117/44‑1 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:1595809/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:190185/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:1833333/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:1196922/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:634214/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:574127/1‑71 (MQ=255) aCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAc > 1:1669155/1‑71 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:977384/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:642885/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:427103/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:1965852/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:1796911/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:1691354/71‑1 (MQ=255) ccGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACg < 1:155409/71‑1 (MQ=255) | CAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACG > W3110S.gb/2172175‑2172304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |