| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,324,489 | C→A | 47.5% | A4S (GCA→TCA) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,324,489 | 0 | C | A | 47.5% | 31.4 / 45.3 | 40 | A4S (GCA→TCA) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (11/10); new base A (11/8); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTC > W3110S.gb/2324426‑2324559 | aCGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGaa < 1:199293/70‑1 (MQ=255)aCGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGaa < 1:1890321/70‑1 (MQ=255)aCGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGaa < 1:77394/70‑1 (MQ=255)aCGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGaa < 1:1836449/70‑1 (MQ=255) aaCGATGTAAAAACCGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAg > 1:279425/1‑70 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc > 1:646274/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc > 1:348388/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc > 1:1766699/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc > 1:153477/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTAAACATGTAgc > 1:1660650/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:682699/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:496511/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:784369/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:1658299/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:835675/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:1039458/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:1415218/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:854823/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:967889/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAgc > 1:1217432/1‑71 (MQ=255) aaCGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATCTa > 1:264404/1‑69 (MQ=255) aGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa < 1:1491877/71‑1 (MQ=255) aGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa < 1:1849206/71‑1 (MQ=255) aGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa < 1:1363819/71‑1 (MQ=255) aGCAATCAACAGCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa < 1:280006/71‑1 (MQ=255) aaCAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGc < 1:1812541/71‑1 (MQ=255) aaCAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGc < 1:300058/71‑1 (MQ=255) aaCAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGc < 1:485516/71‑1 (MQ=255) gCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1555898/71‑1 (MQ=255) gCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1318858/71‑1 (MQ=255) gCCAGAGCACTAACGCCAATGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1471532/71‑1 (MQ=255) gCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTAttt > 1:873215/1‑70 (MQ=255) gCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTAttt > 1:973017/1‑70 (MQ=255) gCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTAttt > 1:18890/1‑70 (MQ=255) gCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTAttt > 1:1048032/1‑70 (MQ=255) cTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1795487/62‑1 (MQ=255) cTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:845298/62‑1 (MQ=255) cTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1401098/62‑1 (MQ=255) ccAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGc > 1:80046/1‑70 (MQ=255) aTGATCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg < 1:1211374/53‑1 (MQ=255) ctctGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTc > 1:162279/1‑71 (MQ=255) ctctGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTc > 1:1026497/1‑71 (MQ=255) | ACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTC > W3110S.gb/2324426‑2324559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |