| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,350,543 | G→A | 40.0% | V337I (GTA→ATA) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,350,543 | 0 | G | A | 40.0% | 26.1 / 44.7 | 40 | V337I (GTA→ATA) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (4/20); new base A (10/6); total (14/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
GAAAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT > W3110S.gb/2350474‑2350601 | gAAAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACgggg > 1:73387/1‑70 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:833218/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:656277/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:1331176/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:1333138/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:1433444/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:1489227/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:233641/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:1673753/70‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa > 1:1358884/1‑71 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa > 1:1456718/1‑71 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa > 1:568629/1‑71 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa > 1:1890965/1‑71 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta < 1:1097863/71‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta < 1:868401/71‑1 (MQ=255) aacaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta < 1:104930/71‑1 (MQ=255) acaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGtat < 1:1153922/71‑1 (MQ=255) acaacCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGtat < 1:571271/71‑1 (MQ=255) tgtgGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta < 1:663406/60‑1 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGtat < 1:234741/54‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa < 1:283827/71‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa < 1:1294307/71‑1 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta > 1:786306/1‑47 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGta > 1:1521593/1‑47 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg > 1:837566/1‑67 (MQ=255) cgcgTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg > 1:727481/1‑67 (MQ=255) gcgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:1794452/71‑1 (MQ=255) gcgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:745771/71‑1 (MQ=255) gcgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:510857/71‑1 (MQ=255) cgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:676946/70‑1 (MQ=255) gtgcgtCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGatat < 1:654513/71‑1 (MQ=255) gtgcgtCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGatat < 1:1938862/71‑1 (MQ=255) tgcgtCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGatat < 1:595065/70‑1 (MQ=255) gtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCAcc < 1:218418/71‑1 (MQ=255) gtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCAcc < 1:1785788/71‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:481064/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:1609918/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:729006/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:1715049/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:1105847/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:1828498/1‑70 (MQ=255) | GAAAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT > W3110S.gb/2350474‑2350601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |