| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,397,191 | A→G | 55.1% | F174S (TTC→TCC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,397,191 | 0 | A | G | 55.1% | 15.2 / 63.9 | 49 | F174S (TTC→TCC) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base G (16/11); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTG > W3110S.gb/2397124‑2397248 | gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACt > 1:998876/1‑71 (MQ=255)gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACt > 1:1901536/1‑71 (MQ=255)gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACt > 1:148258/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:1057865/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:818920/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:731948/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:851721/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:1886693/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:1298922/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:1446638/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg > 1:137392/1‑71 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg > 1:667011/1‑70 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGt < 1:1160631/71‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGt < 1:1454528/71‑1 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATATGCGtt > 1:1550071/1‑71 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtt > 1:1733607/1‑71 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtt > 1:1582083/1‑71 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtt > 1:1551756/1‑71 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg > 1:1347354/1‑70 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc > 1:53851/1‑71 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc > 1:360614/1‑71 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc > 1:281513/1‑71 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc > 1:1945691/1‑71 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1394857/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1231019/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1454936/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1108402/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1131254/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:984262/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1418752/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:783122/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1872206/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1347664/71‑1 (MQ=255) cAGGCCACTCGCCTGGGGGTAAATGAAGAACTTGGTTGCCGCCGt < 1:1067686/45‑1 (MQ=255) gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt < 1:875035/71‑1 (MQ=255) gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt < 1:564587/71‑1 (MQ=255) gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt < 1:370001/71‑1 (MQ=255) cTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCTGTGATACGCGTTTTACCGTCAGAGGCtt < 1:850430/64‑1 (MQ=255) cTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1078145/64‑1 (MQ=255) cTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1787425/64‑1 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGcccc < 1:1971653/69‑1 (MQ=255) ccTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcac < 1:1207333/71‑1 (MQ=255) cTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcac < 1:1102828/70‑1 (MQ=255) gggTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1830876/57‑1 (MQ=255) ggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg > 1:1552273/1‑71 (MQ=255) ggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg > 1:632580/1‑71 (MQ=255) ggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg > 1:1031516/1‑71 (MQ=255) ggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg > 1:1420998/1‑71 (MQ=255) ggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg > 1:1038472/1‑71 (MQ=255) | GTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTG > W3110S.gb/2397124‑2397248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |