| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,426,403 | G→A | 30.8% | G113R (GGG→AGG) | yfcG → | predicted glutathione S‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,426,403 | 0 | G | A | 30.8% | 32.2 / 18.5 | 26 | G113R (GGG→AGG) | yfcG | predicted glutathione S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (6/12); new base A (4/4); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01 | |||||||||||
GAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGT > W3110S.gb/2426334‑2426470 | gAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTgg > 1:1142419/1‑71 (MQ=255) ccACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAAt < 1:1476079/71‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg > 1:1466886/1‑70 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg > 1:1399542/1‑70 (MQ=255) aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:103767/1‑71 (MQ=255) aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCAAGCAg > 1:625528/1‑71 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:1164878/69‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccccc < 1:1004562/57‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccccc < 1:1099/57‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccccc < 1:623222/57‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccccc < 1:340758/57‑1 (MQ=255) aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg > 1:1800831/1‑71 (MQ=255) ggCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCATCCCCCCAAACCATTCCTTACGc < 1:1135597/71‑1 (MQ=255) ggCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc < 1:617888/71‑1 (MQ=255) gCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc < 1:1663647/70‑1 (MQ=255) gCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc < 1:364275/70‑1 (MQ=255) ggACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAt > 1:972832/1‑71 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:316727/58‑1 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:199127/58‑1 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:1666827/58‑1 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:1421177/58‑1 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:845823/58‑1 (MQ=255) gATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:120794/58‑1 (MQ=255) cTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGt > 1:607656/1‑71 (MQ=255) cTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGt > 1:1714203/1‑71 (MQ=255) cTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGt > 1:958986/1‑71 (MQ=255) cTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGt > 1:632951/1‑71 (MQ=255) | GAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGT > W3110S.gb/2426334‑2426470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |