| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,501,495 | G→A | 100% | S135S (TCG→TCA) | ddg → | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,501,495 | 0 | G | A | 85.7% | 48.6 / 2.7 | 21 | S135S (TCG→TCA) | ddg | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (2/16); total (2/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACA > W3110S.gb/2501430‑2501557 | gacaaCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:1589218/68‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:1458185/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:910713/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:591060/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:49370/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:1746517/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:165663/71‑1 (MQ=255) aTAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTAGGCGTCCATTTTATGTCACTGGa < 1:1944823/70‑1 (MQ=255) gcACAAATGCAAAATCGCGGCGTAATTGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGt < 1:1644572/71‑1 (MQ=255) gcACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGt < 1:1911444/71‑1 (MQ=255) gcACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGt < 1:908632/71‑1 (MQ=255) cAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGAc < 1:1858277/70‑1 (MQ=255) cAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGAc < 1:376881/70‑1 (MQ=255) cAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGAc < 1:76396/70‑1 (MQ=255) cAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGAc < 1:131953/70‑1 (MQ=255) aaaaTCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGTGAc < 1:156162/69‑1 (MQ=255) ttGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGt < 1:205942/43‑1 (MQ=255) ttGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGt < 1:1638171/43‑1 (MQ=255) tGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGt < 1:224289/42‑1 (MQ=255) ttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCcaca > 1:174765/1‑70 (MQ=255) ttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCcaca > 1:895434/1‑70 (MQ=255) | GATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACA > W3110S.gb/2501430‑2501557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |