Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,594,104	G→A	100%	H138Y (CAT→TAT)	ypfI ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,594,104	0	G	A	100.0%	31.4 / NA	11	H138Y (CAT→TAT)	ypfI	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/9);  total (2/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAT  >  W3110S.gb/2594054‑2594142                                                   |                                       ttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCg                    >  1:1162059/1‑71 (MQ=255) ttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCg                    >  1:867809/1‑71 (MQ=255)   gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc                   <  1:1752018/70‑1 (MQ=255)   gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc                   <  1:1762880/70‑1 (MQ=255)   gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc                   <  1:30285/70‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:1232743/71‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:1544906/71‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:1567676/71‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:401698/71‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:497730/71‑1 (MQ=255)                   cTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAt  <  1:745620/71‑1 (MQ=255)                                                   |                                       TTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAAT  >  W3110S.gb/2594054‑2594142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑