| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,669,838 | T→C | 100% | V92A (GTT→GCT) | hcaC → | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,669,838 | 0 | T | C | 75.0% | 41.2 / 12.0 | 24 | V92A (GTT→GCT) | hcaC | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base C (9/9); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTC > W3110S.gb/2669773‑2669903 | ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCGAGTACACGTTGaa < 1:909075/70‑1 (MQ=255)ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGaa < 1:1036154/70‑1 (MQ=255)ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGaa < 1:523799/70‑1 (MQ=255)ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGaa < 1:445685/70‑1 (MQ=255)ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGaa < 1:1466904/70‑1 (MQ=255)ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGaa < 1:1791925/70‑1 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGa > 1:1685199/1‑66 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTAc > 1:909048/1‑71 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTAc > 1:43896/1‑71 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTAc > 1:1770659/1‑71 (MQ=255) cctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTCAAGGTGGTGACATTTTCATCGACTTAc > 1:1958218/1‑71 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:1024251/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:1205097/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:616761/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:675442/71‑1 (MQ=255) cGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:1029037/71‑1 (MQ=255) accacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:327695/70‑1 (MQ=255) accacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:1257566/70‑1 (MQ=255) accacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:1059723/70‑1 (MQ=255) tCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:230123/53‑1 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:1791323/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:315663/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:1401417/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:610920/1‑70 (MQ=255) | CCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTC > W3110S.gb/2669773‑2669903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |