| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,697,845 | G→A | 68.9% | G144D (GGT→GAT) | yfhH → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,697,845 | 0 | G | A | 68.9% | 55.6 / 38.0 | 45 | G144D (GGT→GAT) | yfhH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (19/12); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATG > W3110S.gb/2697777‑2697915 | aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTc > 1:108563/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTc > 1:785362/1‑71 (MQ=255) aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGgcg > 1:913533/1‑71 (MQ=255) aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGAGCTTCGGATCTGGTGgcg > 1:1006101/1‑71 (MQ=255) tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:576188/58‑1 (MQ=255) tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:1712823/58‑1 (MQ=255) tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:1780398/58‑1 (MQ=255) gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1506935/71‑1 (MQ=255) gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1618016/71‑1 (MQ=255) gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:238644/71‑1 (MQ=255) gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1909507/71‑1 (MQ=255) gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1793271/71‑1 (MQ=255) cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:1245716/71‑1 (MQ=255) cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:890101/71‑1 (MQ=255) cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:303239/71‑1 (MQ=255) ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:522308/70‑1 (MQ=255) gcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:175101/69‑1 (MQ=255) cggATAATTCTGACCGGTATTTGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGTAAGCTGATGaa < 1:186974/68‑1 (MQ=255) aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:712547/1‑70 (MQ=255) aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:278398/1‑70 (MQ=255) aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:473076/1‑70 (MQ=255) ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:739835/70‑1 (MQ=255) ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:1586889/70‑1 (MQ=255) ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:156095/70‑1 (MQ=255) ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:31029/70‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:566185/70‑1 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAg > 1:1721913/1‑70 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:711262/1‑71 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:713510/1‑71 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:292978/1‑71 (MQ=255) ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1779474/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt > 1:629082/1‑56 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1046611/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:536481/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:869427/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1493772/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1672273/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:732691/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:729654/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1717099/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1846348/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:704698/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:672152/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:382581/1‑71 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:546514/1‑71 (MQ=255) gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:623106/71‑1 (MQ=255) gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:283455/71‑1 (MQ=255) gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:786774/71‑1 (MQ=255) gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:1287653/71‑1 (MQ=255) | ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATG > W3110S.gb/2697777‑2697915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |