| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,710,918 | A→T | 100% | V168E (GTG→GAG) | yfiC ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,710,918 | 0 | A | T | 80.9% | 38.9 / 6.0 | 21 | V168E (GTG→GAG) | yfiC | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (4/0); new base T (2/15); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCG > W3110S.gb/2710873‑2710987 | cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:671476/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:489208/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:127636/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:1475032/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:276793/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:1633557/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:1025400/71‑1 (MQ=255)cTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGt < 1:1871813/71‑1 (MQ=255) aaTGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAAt > 1:292739/1‑71 (MQ=255) aaTGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAAt > 1:1800038/1‑71 (MQ=255) aTGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg < 1:351969/71‑1 (MQ=255) aTGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg < 1:336977/71‑1 (MQ=255) aTGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg < 1:1890281/71‑1 (MQ=255) tGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATg < 1:1298118/70‑1 (MQ=255) ccAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACACAGAAAAATCCCTCTTCGGTAATGCAc > 1:1762977/1‑71 (MQ=255) aCCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGc > 1:1418438/1‑70 (MQ=255) aCCAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGc > 1:1133660/1‑70 (MQ=255) cAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:159183/71‑1 (MQ=255) cAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:130671/71‑1 (MQ=255) cAATCTGCTCTGGCAGCTCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAAc < 1:403859/71‑1 (MQ=255) tGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATgg > 1:1138629/1‑71 (MQ=255) caCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCg < 1:1883934/71‑1 (MQ=255) caCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCg < 1:1484082/71‑1 (MQ=255) | CTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCG > W3110S.gb/2710873‑2710987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |