| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,773,435 | T→C | 22.5% | L488L (TTG→CTG) | yfjW → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,773,435 | 0 | T | C | 22.5% | 77.0 / 20.4 | 40 | L488L (TTG→CTG) | yfjW | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (7/24); new base C (3/6); total (10/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGT > W3110S.gb/2773374‑2773499 | tATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:649404/64‑1 (MQ=255) aTCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:1836685/63‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:802109/62‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:218262/62‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:691845/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:1466892/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:1629733/1‑69 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa > 1:1325782/1‑62 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:433601/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:295766/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:237974/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:316529/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:189871/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:1892757/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:1690732/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:1674581/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:394894/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:146792/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTg < 1:1037002/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTtaata > 1:1414169/1‑70 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTtaa > 1:1218456/1‑68 (MQ=255) aaGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa > 1:1644610/1‑42 (MQ=255) aaGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa > 1:537547/1‑42 (MQ=255) aaGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTa > 1:311855/1‑42 (MQ=255) aaGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATc > 1:51360/1‑70 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:1092121/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:735485/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:1924957/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:140075/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:1385612/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:1357010/70‑1 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGc < 1:135245/70‑1 (MQ=255) atttttTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:1625826/70‑1 (MQ=255) attattTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:1951277/70‑1 (MQ=255) attattTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:472720/70‑1 (MQ=255) attattTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:116473/70‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgt < 1:1493858/71‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgt < 1:448305/71‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgt < 1:1132602/71‑1 (MQ=255) gggAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgt < 1:833515/71‑1 (MQ=255) | TATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGT > W3110S.gb/2773374‑2773499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |