Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,794,102	G→A	100%	L398L (TTG→TTA)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,794,102	0	G	A	87.5%	39.0 / ‑0.2	16	L398L (TTG→TTA)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (2/12);  total (2/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTC  >  W3110S.gb/2794035‑2794126                                                                    |                         taGTCATCGCCGTTTGACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg                        <  1:784399/70‑1 (MQ=255) taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg                        <  1:1413852/70‑1 (MQ=255) taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg                        <  1:1729098/70‑1 (MQ=255)   gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGaa                      <  1:987804/70‑1 (MQ=255)   gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGaa                      <  1:1601664/70‑1 (MQ=255)   gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGaa                      <  1:1674000/70‑1 (MQ=255)   gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGaa                      <  1:619868/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:1321765/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:1486806/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:1576821/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:18564/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:281074/70‑1 (MQ=255)         gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGc                <  1:988921/70‑1 (MQ=255)               tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAAtg                    >  1:898974/1‑60 (MQ=255)               tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAAtg                    >  1:973098/1‑60 (MQ=255)                       gCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGAGAATGTGGCTTTATCCGTGGCTc  <  1:867885/70‑1 (MQ=255)                                                                    |                         TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTC  >  W3110S.gb/2794035‑2794126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑