| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,480 | T→C | 100% | T316A (ACC→GCC) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,480 | 0 | T | C | 81.9% | 49.6 / 4.4 | 22 | T316A (ACC→GCC) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (0/4); new base C (5/13); total (5/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTG > W3110S.gb/2821425‑2821550 | gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCttt > 1:1676177/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt < 1:1052806/70‑1 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt < 1:1631509/70‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:1169103/71‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:695320/71‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:470120/71‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:1943067/71‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGATCTCTTTAGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTAGCAtt < 1:1500407/71‑1 (MQ=255) agcaACTCACGTACTTTCTTCTCGAGCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt < 1:1049856/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:1498110/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:78233/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:680926/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:1742645/71‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:1784337/71‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:1583851/71‑1 (MQ=255) ttcttcTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:1042603/1‑71 (MQ=255) ttcttcTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:910576/1‑71 (MQ=255) ttcttcTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:949961/1‑71 (MQ=255) ttcttcTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAc > 1:495916/1‑70 (MQ=255) gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtctt < 1:457224/69‑1 (MQ=255) gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtctt < 1:1713680/69‑1 (MQ=255) gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtctt < 1:702527/69‑1 (MQ=255) tttCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTg < 1:1918383/71‑1 (MQ=255) tttCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTg < 1:1643936/71‑1 (MQ=255) | GAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTG > W3110S.gb/2821425‑2821550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |