| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,867,123 | G→A | 100% | P96L (CCG→CTG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,867,123 | 0 | G | A | 86.7% | 78.5 / 4.0 | 30 | P96L (CCG→CTG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (10/16); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAA > W3110S.gb/2867063‑2867177 | ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:1034259/71‑1 (MQ=255)ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:695340/71‑1 (MQ=255)ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:621069/71‑1 (MQ=255)ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:1581000/71‑1 (MQ=255)ccTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGc < 1:1031823/71‑1 (MQ=255) tGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGc < 1:873983/71‑1 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1618522/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:140122/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1463462/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1515649/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGa > 1:1946738/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTCCACTGGCTGGa > 1:1954764/1‑71 (MQ=255) cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACAGGCTGCTGAGCTACTGGCTGCACAGGCTGGa > 1:368965/1‑71 (MQ=255) tGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGatca > 1:125010/1‑68 (MQ=255) tGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGatca > 1:1541581/1‑68 (MQ=255) tGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg > 1:930043/1‑69 (MQ=255) tGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg > 1:922924/1‑69 (MQ=255) tGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg > 1:109843/1‑69 (MQ=255) tGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAg > 1:1497106/1‑69 (MQ=255) tGCGTCCGTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGATGAg > 1:1166109/1‑69 (MQ=255) gTTTTCCATCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGAGGTTGTTGAGTAGCCTGaa < 1:1084443/71‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTTGCTGCACTGGCTGGATTTGCg < 1:1353369/45‑1 (MQ=38) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGTTTTGCg < 1:330/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:1931941/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:1522663/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:587448/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:1445590/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:711060/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:1384966/45‑1 (MQ=255) tCTGTACCAGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCg < 1:917135/45‑1 (MQ=255) | CCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAA > W3110S.gb/2867063‑2867177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |