| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,893,179 | A→C | 100% | I110L (ATT→CTT) | ygcP → | predicted anti‑terminator regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,893,179 | 0 | A | C | 92.9% | 96.2 / ‑2.3 | 28 | I110L (ATT→CTT) | ygcP | predicted anti‑terminator regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (19/7); total (21/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGA > W3110S.gb/2893116‑2893237 | aCCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGa > 1:356054/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTa > 1:1390162/1‑49 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTa > 1:1755111/1‑49 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTa > 1:142823/1‑49 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTa > 1:1431179/1‑49 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTa > 1:1448311/1‑49 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:653113/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:1894968/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:389433/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:422812/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:1640825/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:1410234/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:845591/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:1363869/1‑71 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:858176/1‑71 (MQ=255) aaaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:582896/71‑1 (MQ=255) aaaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:528708/71‑1 (MQ=255) aaaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:470408/71‑1 (MQ=255) aaaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:103792/71‑1 (MQ=255) ggCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTg < 1:1105084/71‑1 (MQ=255) ggCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTg < 1:1325456/71‑1 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAg > 1:202264/1‑70 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAg > 1:1757362/1‑70 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAg > 1:29483/1‑70 (MQ=255) ggTTTTTTCTGTATTCATCTCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:1574615/53‑1 (MQ=255) tttttCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTgcgc > 1:377069/1‑70 (MQ=255) tttttCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTgcgc > 1:187508/1‑70 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGGAAGTTGCGCAATCGAATCCGGa > 1:378029/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:1899255/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:1710816/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:1562117/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:1493277/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:1374679/1‑71 (MQ=255) | ACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGA > W3110S.gb/2893116‑2893237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |