| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,916,386 | G→A | 100% | E892K (GAA→AAA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,916,386 | 0 | G | A | 77.8% | 49.4 / 12.0 | 27 | E892K (GAA→AAA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (6/15); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
CGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGC > W3110S.gb/2916332‑2916450 | cGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAAcc > 1:1126026/1‑71 (MQ=255) tATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCgg < 1:442949/71‑1 (MQ=255) tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGctgctg > 1:194725/1‑59 (MQ=255) tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc > 1:111141/1‑71 (MQ=255) tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc > 1:1674602/1‑71 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc > 1:1058682/1‑69 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc > 1:852775/1‑69 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc > 1:1296899/1‑69 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:820219/71‑1 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:1955416/71‑1 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCAGGAACTGTTGGACga < 1:480987/71‑1 (MQ=255) tCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:133056/70‑1 (MQ=255) tCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:1101086/70‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1399222/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:903439/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:595040/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1849722/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:396484/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:230063/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1836496/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:157657/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1544058/71‑1 (MQ=255) gCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1514287/67‑1 (MQ=255) tACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:895244/48‑1 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc > 1:1053581/1‑70 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc > 1:67827/1‑70 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc > 1:932247/1‑70 (MQ=255) | CGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGC > W3110S.gb/2916332‑2916450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |