| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,938,519 | A→G | 17.0% | I166V (ATC→GTC) | fucR → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,938,519 | 0 | A | G | 17.0% | 125.9 / 19.7 | 53 | I166V (ATC→GTC) | fucR | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (20/24); new base G (5/4); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
ACACTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCG > W3110S.gb/2938453‑2938584 | acacTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGa < 1:1779327/71‑1 (MQ=255)acacTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGa < 1:89365/71‑1 (MQ=255)acacTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGa < 1:55757/71‑1 (MQ=255) tatGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGa < 1:890237/56‑1 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1159041/1‑71 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1424361/1‑71 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:708855/1‑71 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAgg > 1:298508/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAgg > 1:430497/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1360229/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAgg > 1:711232/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTAtttt > 1:1454037/1‑58 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1859329/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:476939/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:478032/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1447832/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1211115/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:921971/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:1157786/1‑70 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:957635/1‑70 (MQ=255) aCGTCAATCCCTCGATGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAgg > 1:986601/1‑70 (MQ=255) cGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAggg < 1:1819565/70‑1 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:963784/1‑71 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:882051/71‑1 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc > 1:661370/1‑71 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:1679907/71‑1 (MQ=255) aTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:1933925/60‑1 (MQ=255) aTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:1175173/60‑1 (MQ=255) aTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:614250/60‑1 (MQ=255) tCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:227189/59‑1 (MQ=255) tCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:45543/59‑1 (MQ=255) tCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTagtg < 1:1492533/59‑1 (MQ=255) cccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTtgtg < 1:1741506/58‑1 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:1892782/1‑70 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:1374902/1‑70 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:175186/1‑70 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:413462/1‑70 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:38116/1‑70 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcg > 1:928790/1‑71 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:19970/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:770510/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:912372/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:1344308/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:1193180/70‑1 (MQ=255) aaCTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg < 1:607342/71‑1 (MQ=255) aCTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:824376/62‑1 (MQ=255) tCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGTGATCGATAGCAGCGGCGCa < 1:353163/57‑1 (MQ=255) tCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCa < 1:1485816/57‑1 (MQ=255) tCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCa < 1:1865042/57‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCg < 1:496516/69‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCg < 1:646903/69‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCg < 1:1106459/69‑1 (MQ=255) gAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCg < 1:1009485/69‑1 (MQ=255) | ACACTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCCAATGCG > W3110S.gb/2938453‑2938584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |