| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,013,681 | G→A | 59.6% | V11V (GTC→GTT) | yqeC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,013,681 | 0 | G | A | 59.6% | 29.4 / 61.5 | 52 | V11V (GTC→GTT) | yqeC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/9); new base A (12/19); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
CACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTA > W3110S.gb/3013632‑3013743 | cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:988823/1‑70 (MQ=255)cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:697898/1‑70 (MQ=255)cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:618303/1‑70 (MQ=255)cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:363060/1‑70 (MQ=255)cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:332210/1‑70 (MQ=255)aaCCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAAc > 1:1963334/2‑70 (MQ=255) ccAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACta > 1:317055/1‑70 (MQ=255) ccAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACta > 1:330685/1‑70 (MQ=255) acaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACtttt > 1:54557/1‑71 (MQ=255) acaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACttt > 1:1064891/1‑70 (MQ=255) acaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:882180/71‑1 (MQ=255) acaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:794196/71‑1 (MQ=255) acaGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:354171/71‑1 (MQ=255) aTCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGa > 1:1958915/1‑70 (MQ=255) aTCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAg > 1:1625049/1‑71 (MQ=255) aTCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAg > 1:1882367/1‑71 (MQ=255) aTCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAg > 1:1039698/1‑71 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:333803/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:1651474/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:1771640/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:869489/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:294108/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:997427/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:1188756/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:1764295/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCttt < 1:1586092/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:666200/70‑1 (MQ=255) ggACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:103523/56‑1 (MQ=255) gACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:1114283/71‑1 (MQ=255) gACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:986504/71‑1 (MQ=255) gACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:1640933/71‑1 (MQ=255) gACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:7376/71‑1 (MQ=255) aCGTTTCTGCGCACCTAAATCAATGACTAATGCAGATGGGTCAACTATACTTTTCACGAGTCttt < 1:1243444/65‑1 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACTAGTCTTTATGACctct > 1:352217/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct > 1:602087/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct > 1:810298/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct > 1:1570791/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct > 1:1593717/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct > 1:313543/1‑71 (MQ=255) tttCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctc > 1:1226413/1‑70 (MQ=255) cTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt < 1:335244/71‑1 (MQ=255) cTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt < 1:1818313/71‑1 (MQ=255) cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:1919503/56‑1 (MQ=255) cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:504405/56‑1 (MQ=255) cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:82325/56‑1 (MQ=255) cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:1175705/56‑1 (MQ=255) cTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:93137/56‑1 (MQ=255) tAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt > 1:1626177/1‑70 (MQ=255) tAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt > 1:1789902/1‑70 (MQ=255) tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc < 1:1538313/55‑1 (MQ=255) aaaTCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa > 1:219641/1‑71 (MQ=255) aTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAtt < 1:790295/68‑1 (MQ=255) | CACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTA > W3110S.gb/3013632‑3013743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |