| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,205,103 | A→G | 79.4% | intergenic (‑191/‑16) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,205,103 | 0 | A | G | 79.4% | 72.4 / 14.1 | 34 | intergenic (‑191/‑16) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base G (4/23); total (9/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
GGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG > W3110S.gb/3205035‑3205166 | ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:784870/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:475560/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:1665260/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:1641918/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:1836073/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:1422614/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:1017093/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:338910/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 1:772821/71‑1 (MQ=255) aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:1057243/71‑1 (MQ=255) aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:778681/71‑1 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:1360720/70‑1 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGc < 1:477804/71‑1 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGc < 1:1544763/71‑1 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGc < 1:1032986/71‑1 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat < 1:603952/70‑1 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGc < 1:1175684/60‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1881223/70‑1 (MQ=255) tAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:104091/43‑1 (MQ=255) tAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:1041483/43‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:1812859/70‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:973864/70‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:737274/70‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:657406/70‑1 (MQ=255) ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:224321/1‑70 (MQ=255) ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:1645233/1‑70 (MQ=255) ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:1035002/1‑70 (MQ=255) ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:1351309/1‑70 (MQ=255) ttGTGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:1034936/1‑70 (MQ=255) ggTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt < 1:1444631/71‑1 (MQ=255) gTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt < 1:476403/70‑1 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:312452/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:424963/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:1259198/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:959004/1‑70 (MQ=255) cAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa < 1:661133/70‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga < 1:235359/71‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga < 1:855064/71‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga < 1:1951869/71‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga < 1:117922/71‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga < 1:1270548/71‑1 (MQ=255) cAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:194722/71‑1 (MQ=255) cAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:613953/71‑1 (MQ=255) | GGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG > W3110S.gb/3205035‑3205166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |