| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,219,258 | T→C | 29.0% | F379S (TTC→TCC) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,219,258 | 0 | T | C | 29.0% | 42.5 / 21.7 | 31 | F379S (TTC→TCC) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (14/8); new base C (3/6); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGG > W3110S.gb/3219191‑3219312 | aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCg > 1:1104355/1‑71 (MQ=255)aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCg > 1:1714612/1‑71 (MQ=255) gCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCggg < 1:1086526/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:1161947/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:1415637/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:1506809/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:735812/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:1663716/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:582596/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:176169/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:338094/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:283831/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:589474/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:1801230/71‑1 (MQ=255) aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:694663/71‑1 (MQ=255) gAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa > 1:373785/1‑70 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat > 1:1610783/1‑71 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat > 1:1107028/1‑71 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat > 1:548440/1‑71 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat > 1:481924/1‑71 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAata > 1:310033/1‑70 (MQ=255) ccGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCg < 1:791982/70‑1 (MQ=255) aaGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGt > 1:748056/1‑71 (MQ=255) aaGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGt > 1:125959/1‑71 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:47832/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:1942193/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:1824034/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:595332/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:626924/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:1787257/1‑70 (MQ=255) tGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:1521233/1‑70 (MQ=255) | AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGG > W3110S.gb/3219191‑3219312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |