| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,311,878 | G→A | 40.0% | P252L (CCG→CTG) | truB ← | tRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,311,878 | 0 | G | A | 40.0% | 25.7 / 44.8 | 40 | P252L (CCG→CTG) | truB | tRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base G (7/17); new base A (11/5); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
CGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGT > W3110S.gb/3311821‑3311925 | cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc > 1:574104/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc > 1:1066116/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc > 1:844683/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc > 1:1769137/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGc > 1:1415271/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCAGAAg > 1:124734/1‑70 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGc > 1:496119/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGc > 1:763683/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGc > 1:1273623/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGc > 1:1317058/1‑71 (MQ=255)cGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGc > 1:807276/1‑71 (MQ=255) aaCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTg < 1:1024664/71‑1 (MQ=255) aaCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTg < 1:1422949/71‑1 (MQ=255) aaCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTg < 1:789196/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:1402386/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:1542982/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:1584285/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:1868219/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:824948/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:207440/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:210644/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:341348/71‑1 (MQ=255) aCCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTgg < 1:142810/71‑1 (MQ=255) ccGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTgg < 1:192108/70‑1 (MQ=255) ttACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTc < 1:1162290/71‑1 (MQ=255) aGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGt < 1:75312/71‑1 (MQ=255) aaCAGAAGAGGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:240692/1‑71 (MQ=255) aaCAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:768854/1‑71 (MQ=255) aaCAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:1880797/1‑71 (MQ=255) aaCAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:1018553/1‑71 (MQ=255) aaCAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:1541043/1‑71 (MQ=255) aaCAGAAGACGTTAACGGAAGATTCACCACCAGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATg > 1:1206210/1‑71 (MQ=255) aCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:576838/71‑1 (MQ=255) aCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:1382327/71‑1 (MQ=255) aCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:8707/71‑1 (MQ=255) cGTTAACGTAAGATTCACCACCGGATAGTCCGAAGCTgg < 1:1079333/39‑1 (MQ=255) cGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:702198/70‑1 (MQ=255) cGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:282856/70‑1 (MQ=255) gTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGt < 1:1392227/69‑1 (MQ=255) aaCGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTaa > 1:622345/1‑56 (MQ=255) | CGAACCGGGTTACCATTTTTGAAGTAAACAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGT > W3110S.gb/3311821‑3311925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |