Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,649,364	Δ1 bp	coding (1580/4962 nt)	yfhM ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,649,361	0	A	.	100.0%	68.2 / NA	17	coding (1583/4962 nt)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/17);  total (0/17)

TCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA  >  W3110S.gb/2649316‑2649381                                              |                     tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1572647/65‑1 (MQ=255) tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:894369/65‑1 (MQ=255) tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:449596/65‑1 (MQ=255) tCAAAACCACGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:326011/65‑1 (MQ=255)             aaGGCTGACACAGCTTCACTCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1005029/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAGAGTa  <  1:2687136/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:898863/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:837845/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:562333/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:456873/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2975260/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2393296/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2240500/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2217163/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1900571/53‑1 (MQ=255)             aaGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1302572/53‑1 (MQ=255)              aGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1691610/52‑1 (MQ=255)                                              |                     TCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA  >  W3110S.gb/2649316‑2649381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑