Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,897,815 Δ1 bp coding (260/1455 nt) ygcU ← predicted FAD containing dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,897,8140T.100.0% 60.4 / NA 15coding (261/1455 nt)ygcUpredicted FAD containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/3);  total (12/3)

TGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTC  >  W3110S.gb/2897750‑2897874
                                                                |                                                            
tgatttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCt                                                              >  1:458303/1‑65 (MQ=255)
tgatttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCt                                                              >  1:1860552/1‑65 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGc                                                >  1:744435/1‑64 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGc                                                >  1:119560/1‑64 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGCa                                               >  1:179754/1‑65 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGCa                                               >  1:2353589/1‑65 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGCa                                               >  1:2203068/1‑65 (MQ=255)
              gCGGAGCCGTCGAGCACCACCGAGTTTTATACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGc                                                >  1:1213937/1‑64 (MQ=255)
                       tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCt                                                              >  1:1783502/1‑42 (MQ=255)
                       tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGCAGCACCGGTAcg                                       >  1:215980/1‑64 (MQ=255)
                       tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGCACCGGTAcgc                                      >  1:2857888/1‑65 (MQ=255)
                       tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTCGGTGGCGGAAGCACCGGTAcgc                                      >  1:2135432/1‑65 (MQ=255)
                                            acaacaGTTTCCAGCCCACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTtgtg                  >  1:2095955/1‑64 (MQ=255)
                                            acaacaGTTTCCAGCCCACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTtgtg                  >  1:1975982/1‑64 (MQ=255)
                                            acaacaGTTTCCAGCCCACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTtgtg                  >  1:1952590/1‑64 (MQ=255)
                                                         gCCCACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAAt    <  1:1267862/65‑1 (MQ=255)
                                                           ccACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTc  <  1:1594235/65‑1 (MQ=255)
                                                           ccACCTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTc  <  1:821725/65‑1 (MQ=255)
                                                                |                                                            
TGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTC  >  W3110S.gb/2897750‑2897874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: