Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	547,541	(A)6→5	coding (1638/1668 nt)	fdrA →	predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	547,536	0	A	.	100.0%	15.3 / NA	5	coding (1633/1668 nt)	fdrA	predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/0);  total (5/0)

AGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAG  >  W3110S.gb/547482‑547593                                                       |                                                          aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:128630/1‑57 (MQ=255) aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:1654388/1‑57 (MQ=255) aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:2857603/1‑57 (MQ=255) aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:2898199/1‑57 (MQ=255) aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:743371/1‑57 (MQ=255) aGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATaaa                                                         >  1:920526/1‑57 (MQ=255)                              aTGGTCGCCAGTCGCCGGTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGt                   >  1:1279122/1‑65 (MQ=255)                              aTGGTCGCCAGTCGCCGGTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGt                   >  1:1720500/1‑65 (MQ=255)                              aTGGTCGCCAGTCGCCGGTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGt                   >  1:2029521/1‑65 (MQ=255)                              aTGGTCGCCAGTCGCCGGTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGt                   >  1:634709/1‑65 (MQ=255)                                               gTGGCAAT‑AAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAg  >  1:1771284/1‑65 (MQ=255)                                                       |                                                          AGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAG  >  W3110S.gb/547482‑547593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑