Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,395,175	(T)5→4	coding (1017/1053 nt)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,395,171	0	T	.	100.0%	28.6 / NA	8	coding (1021/1053 nt)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/0);  total (8/0)

ACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCA  >  W3110S.gb/4395120‑4395185                                                    |               acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:1235114/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:1719810/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:222576/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:252783/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:362041/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:786615/1‑65 (MQ=255) acgacgGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:949066/1‑65 (MQ=255) acgacgGTTAGCTTAATTCTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCa  >  1:761190/1‑65 (MQ=255)                                                    |               ACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCA  >  W3110S.gb/4395120‑4395185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑