Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,027,474	(T)7→6	intergenic (+124/‑174)	yodD → / → yedP	hypothetical protein/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,027,468	0	T	.	100.0%	69.7 / NA	17	intergenic (+118/‑180)	yodD/yedP	hypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/11);  total (6/11)

TCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAT  >  W3110S.gb/2027453‑2027524                |                                                         tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:3040915/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2866142/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2752762/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2056239/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2268169/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2373320/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2483939/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2528740/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2698504/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt         <  1:2690721/64‑1 (MQ=255) tcaCCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATAATCGCCATATGAATGATTAACGCTACt         <  1:1866249/64‑1 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:2587888/1‑64 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:1933791/1‑64 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:1866358/1‑64 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:1597941/1‑64 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:414425/1‑64 (MQ=255)        aTCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAt  >  1:49350/1‑64 (MQ=255)                |                                                         TCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAAT  >  W3110S.gb/2027453‑2027524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑