Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,036,331	(C)5→6	coding (432/1734 nt)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,036,326	1	.	C	100.0%	27.1 / NA	9	V146G (GTT→GGT)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/9);  total (0/9)

CCCAACGAGCGAGCGTGCTCAA‑CCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACA  >  W3110S.gb/3036305‑3036364                       |                                       cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:1183381/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:1558294/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:2720250/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:2760919/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:2866757/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:513702/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:828324/61‑1 (MQ=255) cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACAGTGACa  <  1:1175843/61‑1 (MQ=255) cccAACGAGAGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa  <  1:910074/61‑1 (MQ=255)                       |                                       CCCAACGAGCGAGCGTGCTCAA‑CCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACA  >  W3110S.gb/3036305‑3036364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑