Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,359,804	+A	coding (752/1419 nt)	gltD →	glutamate synthase, 4Fe‑4S protein, small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,359,800	1	.	A	100.0%	108.1 / NA	35	E250K (GAA→AAA)	gltD	glutamate synthase, 4Fe‑4S protein, small subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (35/0);  total (35/0)

TGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAAC  >  W3110S.gb/3359738‑3359853                                                                |                                                      tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a                                                      >  1:1120614/1‑64 (MQ=255) tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a                                                      >  1:247394/1‑64 (MQ=255) tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a                                                      >  1:1011501/1‑64 (MQ=255) tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a                                                      >  1:1887626/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGAc                                            >  1:2581997/1‑63 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:912464/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:2473987/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:239403/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:2345795/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:2258632/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:3081993/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:596314/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:2004781/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1915618/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1882452/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1795105/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:633862/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1351064/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1294650/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:1290767/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg                                           >  1:635963/1‑64 (MQ=255)             aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGC‑GGCTGG‑aa                                                     >  1:2975891/1‑52 (MQ=255)                                            gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc           >  1:1859243/1‑65 (MQ=255)                                            gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc           >  1:628751/1‑65 (MQ=255)                                            gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc           >  1:2371626/1‑65 (MQ=255)                                            gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc           >  1:1266785/1‑65 (MQ=255)                                                  gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc     >  1:2264460/1‑65 (MQ=255)                                                  gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc     >  1:2861830/1‑65 (MQ=255)                                                  gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc     >  1:2852426/1‑65 (MQ=255)                                                  gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc     >  1:2196748/1‑65 (MQ=255)                                                  gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc     >  1:1054047/1‑65 (MQ=255)                                                     cggtggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:2862863/1‑65 (MQ=38)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc           >  1:2848892/1‑56 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCaa   >  1:1670944/1‑64 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:2692533/1‑65 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:2183566/1‑65 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:442160/1‑65 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:2175579/1‑65 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:1812244/1‑65 (MQ=255)                                                     cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac  >  1:1143153/1‑65 (MQ=255)                                                                |                                                      TGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAAC  >  W3110S.gb/3359738‑3359853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑