| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,493,301 | +A | coding (851/1080 nt) | frwC ← | predicted enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,493,298 | 1 | . | A | 96.5% | 91.4 / ‑2.8 | 29 | coding (854/1080 nt) | frwC | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (7/21); total (7/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
ATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGG > W3110S.gb/3493238‑3493356 | aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:669066/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:2205349/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1974138/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:748025/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1018464/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1879189/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:2323646/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1676493/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1665669/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:475993/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:870644/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:1370544/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:2905672/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:3015652/65‑2 (MQ=255)aTCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:3071385/65‑2 (MQ=255) ccTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatga > 1:1607427/1‑65 (MQ=255) ccTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCatga > 1:1733483/1‑65 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:404254/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:2891641/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:2495679/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:448361/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:1893749/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:1556570/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:1337969/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATgg < 1:1049367/65‑1 (MQ=255) gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAATCTGAACCGACCATGATgg < 1:208341/65‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaag < 1:2263609/65‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaag < 1:1539747/65‑1 (MQ=255) tAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaag < 1:967619/65‑1 (MQ=255) aGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaag < 1:844917/64‑1 (MQ=255) aCTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑aaaa < 1:41033/40‑2 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg > 1:1848409/1‑60 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg > 1:1787999/1‑60 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg > 1:2844985/1‑60 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg > 1:112459/1‑60 (MQ=255) tGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAA‑CTGAACCGACCATGATggaagg > 1:3011527/1‑60 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:2288707/65‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:299313/65‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:1951217/65‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:1614943/65‑1 (MQ=255) aCTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:1538170/65‑1 (MQ=255) cTGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGa < 1:347185/64‑1 (MQ=255) tGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGaa < 1:2799743/64‑1 (MQ=255) tGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGaa < 1:2605438/64‑1 (MQ=255) tGCACCACAAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGaa < 1:940120/64‑1 (MQ=255) caccacaAAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:1043150/1‑65 (MQ=255) | ATCAGACCACCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAA‑CTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGG > W3110S.gb/3493238‑3493356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |