| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,434,329 | +A | intergenic (+92/‑217) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,434,326 | 1 | . | A | 85.7% | 35.3 / 1.7 | 14 | intergenic (+89/‑220) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (0/12); total (0/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TTGTGTTGC‑AAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACA > W3110S.gb/4434318‑4434385 | ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:1116357/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:1170158/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:1259028/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:1494598/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:1848779/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:2018931/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:2462523/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:600233/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:611157/63‑1 (MQ=255)ttGTGTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGAGTTATCACCGTGat < 1:1185788/63‑1 (MQ=255) tgtgTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:2191630/62‑1 (MQ=255) tgtgTTGCAAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGat < 1:429671/62‑1 (MQ=255) gtTGC‑AAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGaca < 1:1748062/64‑1 (MQ=255) gtTGC‑AAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGaca < 1:454050/64‑1 (MQ=255) | TTGTGTTGC‑AAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACA > W3110S.gb/4434318‑4434385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |