Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,452,302	+T	coding (3509/3780 nt)	ytfN →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,452,299	1	.	T	100.0%	37.0 / NA	13	G1169V (GGT→GTT)	ytfN	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (13/0);  total (13/0)

ACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGG‑TTTGGGGGTTGC  >  W3110S.gb/4452237‑4452311                                                                |             aCGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGG‑tt            >  1:41861/1‑65 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:1118492/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:1244552/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:1299178/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:158152/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:2041028/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:2205196/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:2507749/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:443252/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTgc  >  1:552328/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTg   >  1:1552673/1‑63 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTg   >  1:229619/1‑63 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTGGGGGTTg   >  1:2926770/1‑63 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTTCGGGGTTgc  >  1:1954135/1‑64 (MQ=255)             gCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGG‑tt            >  1:1861394/1‑53 (MQ=255)                                                                |             ACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGG‑TTTGGGGGTTGC  >  W3110S.gb/4452237‑4452311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑