Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,014,752	(TTG)2→3	coding (1072/1641 nt)	pqiB →	paraquat‑inducible protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,014,744	1	.	T	77.8%	15.8 / 2.0	9	coding (1064/1641 nt)	pqiB	paraquat‑inducible protein B
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (0/7);  total (0/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
*	W3110S.gb	1,014,744	2	.	G	77.8%	21.7 / 1.2	9	coding (1064/1641 nt)	pqiB	paraquat‑inducible protein B
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (0/7);  total (0/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	W3110S.gb	1,014,744	3	.	T	77.8%	15.8 / 2.0	9	coding (1064/1641 nt)	pqiB	paraquat‑inducible protein B
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (0/7);  total (0/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGA‑‑‑TGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGC  >  W3110S.gb/1014706‑1014794                                        |||                                                   gCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGA‑‑‑TGTTGTTGAGCACCTTGGCGAAttg                           <  1:1866450/64‑1 (MQ=255) gCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGA‑‑‑TGTTGTTGAGCACCTTGGCGAAttg                           <  1:742929/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:1490249/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:1706202/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:2047934/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:2785338/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:2870920/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:3014621/64‑1 (MQ=255)                             gAAAATGCGGATGTTGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGc  <  1:363775/64‑1 (MQ=255)                                        |||                                                   GCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGA‑‑‑TGTTGTTGAGCACCTTGGCGAATTGTTGAAACGTGGTTTACGCGGATCGC  >  W3110S.gb/1014706‑1014794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑