BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	160,958	(A)_5→6	coding (529/705 nt)	sfsA ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	160,953	1	.	A	100.0%	37.5 / NA	13	F178F (TTC→TTT)	sfsA	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (6/7); total (6/7)

TATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑AAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAGG  >  W3110S.gb/160894‑161007

tATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   <  1:43837/65‑1 (MQ=255)

tATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   <  1:719828/65‑1 (MQ=255)

tATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   <  1:2708229/65‑1 (MQ=255)

tATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   <  1:1597896/65‑1 (MQ=255)

tATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   <  1:1615836/65‑1 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:899048/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:680210/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:412532/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:3095950/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:100331/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:2333151/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:2213633/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:733261/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:737308/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:1932360/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:1913684/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:767207/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:1363060/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:1218872/62‑2 (MQ=255)

    tctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:1208924/62‑2 (MQ=255)

     ctcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaaa                                                  <  1:626758/61‑2 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:852438/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:2617103/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:1488299/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:2999717/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:1312602/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCGAAAAAAGATaa                                              >  1:714379/1‑65 (MQ=255)

      tcATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑aaaaa                                                   >  1:3027230/1‑59 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:2358148/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:313105/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:2159788/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:1947952/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:1753097/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:1589262/65‑1 (MQ=255)

                                                  cagcaCGGCGAAAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAgg  <  1:1174021/65‑1 (MQ=255)

TATTTCTCATCGATGTGGCGCGCGGGTGAAAACCGTGTAATGGCTGAATGCAGCACGGCG‑AAAAAGATAACCGCACGCTGGCCTTCAGCCGCTACGCTCATCAACTCCCGAAGG  >  W3110S.gb/160894‑161007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`