Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,482,576	(T)5→6	coding (1725/1758 nt)	ynbC →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,482,571	1	.	T	87.5%	18.6 / ‑2.1	8	coding (1720/1758 nt)	ynbC	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (7/0);  total (8/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTA‑TTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGT  >  W3110S.gb/1482514‑1482613                                                           |                                           tCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTA‑TTTTTAc                                     >  1:1984737/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:1051999/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:1301120/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:1370370/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:1678791/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:2325237/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:235422/1‑65 (MQ=255)                                     caACGGATTGATGAGTGGGGTATTTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGt  >  1:2840924/1‑65 (MQ=255)                                                           |                                           TCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTA‑TTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGT  >  W3110S.gb/1482514‑1482613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑