| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,491,334 | +A | coding (1389/1440 nt) | aldA → | aldehyde dehydrogenase A, NAD‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,491,331 | 1 | . | A | 87.5% | 43.8 / 1.5 | 16 | coding (1386/1440 nt) | aldA | aldehyde dehydrogenase A, NAD‑linked |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (0/14); total (0/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑AAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAG > W3110S.gb/1491281‑1491379 | cAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑AAACATGGCTTg < 1:2834885/63‑1 (MQ=255)cAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑AAACATGGCTTg < 1:1650581/63‑1 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:425383/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:2668523/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:2666727/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:2991091/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:307350/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:2082864/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:346266/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:1854883/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:685595/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:1486667/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:1340416/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:738876/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aaa > 1:1237159/1‑46 (MQ=255) ccACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑aa > 1:1150122/1‑45 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:647036/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2244765/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:319071/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:750569/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2694663/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2340356/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2115807/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2112222/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:2021042/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:1947515/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:191072/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:1734219/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:1247247/64‑1 (MQ=255) ggcggcGCAGATGGTAAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAg < 1:1152576/64‑1 (MQ=255) | CAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGT‑AAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAG > W3110S.gb/1491281‑1491379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |