Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,549,446	+C	coding (332/3048 nt)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,549,443	1	.	C	75.0%	63.4 / 16.8	24	coding (329/3048 nt)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base C (18/0);  total (18/6)
Fisher's exact test for biased strand distribution p-value = 7.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCG  >  W3110S.gb/1549404‑1549482                                         |                                        gTTTGCTGGATTACGTCAACAGTGAGAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1116599/65‑1 (MQ=255) gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:2426692/65‑1 (MQ=255) gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:2447492/65‑1 (MQ=255) gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1840771/65‑1 (MQ=255) gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1224485/65‑1 (MQ=255)  tttGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1376149/64‑1 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2598934/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:989369/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:832667/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:703736/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:684063/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:572060/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:473869/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:359382/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:3050421/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1198711/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2346669/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2186600/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2138646/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1839903/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1740318/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1704056/1‑65 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgc   >  1:1072978/1‑64 (MQ=255)                tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgc   >  1:68291/1‑64 (MQ=255)                                         |                                        GTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCG  >  W3110S.gb/1549404‑1549482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑